Buyer	Royal Botanic Gardens, Kew — not a council we hold
Supplier	Novogene (UK) Company LTD

Royal Botanic Gardens, Kew wishes to appoint a supplier to individually sequence the whole genomes of up to 300 European ash trees (Fraxinus excelsior) at a minimum of 15x whole genome coverage via a long-read technology. Fraxinus excelsior is a diploid and has a 1C genome size of 880 Mbp. In addition to the 300 individual ash trees, we wish to sequence a minimum of six technical replicates. Therefore, the total number of samples we wish to sequence is 306. RBG Kew intends to supply total genomic DNA for each sample with the following metrics: minimum of 10ug DNA; minimum concentration of 30ng/ml (as measured with a Quantus flurometer dsDNA assay); at least 50% of the sample in fragments with a minimum length of 40kb; minimum OD260/280 of 1.8; minimum OD260/230 of 1.8; minimum DIN of 7.

Runs	19 Aug 2024 to 18 Aug 2025
OCID	ocds-b5fd17-f0a5cd0e-71a2-47f9-a409-e20abaae72f9
Notice	Read it on Contracts Finder

Figures are as published An award value in OCDS may be a framework ceiling rather than a sum anyone expects to spend, so a large figure here is not evidence of a large purchase.